Journal: Immunity

Article Title: LAG-3 Inhibitory Receptor Expression Identifies Immunosuppressive Natural Regulatory Plasma Cells

doi: 10.1016/j.immuni.2018.06.007

Figure Lengend Snippet:

Article Snippet: A-tailing was performed using 5U Klenow exo - (New England Biolabs), and Illumina TruSeq adaptors were ligated at 16°C overnight using 2000U T4-Ligase (New England Biolabs).

Techniques: Cell Isolation, Recombinant, Electron Microscopy, Staining, SYBR Green Assay, DNA Methylation Assay, Expressing, Labeling, Sample Prep, Methylation, Purification, Mutagenesis, Plasmid Preparation, Transfection

Journal: Molecular cell

Article Title: Transcription Factor-Directed Re-Wiring of Chromatin Architecture for Somatic Cell Nuclear Reprogramming Toward Trans-differentiation

doi: 10.1016/j.molcel.2019.07.036

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Ligation was performed in 1.2ml by adding mQ water, T4 DNA ligase buffer to concentration 1X (NEB, B0202), 0.083% TritionX-100, 0.01mg/mL BSA, 2000U/uL T4 DNA Ligase (NEB, M0202) for 4 hr at RT with slow rotation.

Techniques: Recombinant, Transfection, Bicinchoninic Acid Protein Assay, Plasmid Preparation, Software, Microscopy